openslide_read_region filling buffer with 0's for Mirax

PD Dr. med. M. Weihrauch m.weihrauch at smartinmedia.com
Fri Apr 10 04:23:32 EDT 2020


3dHistech can also give you there SDK. it is somewhat buggy, but reads all files..

best

Martin



Von meinem Samsung Galaxy Smartphone gesendet.



-------- Ursprüngliche Nachricht --------
Von: "T. N." <ca95129 at hotmail.com>
Datum: 10.04.20 10:16 (GMT+01:00)
An: openslide-users at lists.andrew.cmu.edu
Betreff: Re: openslide_read_region filling buffer with 0's for Mirax


I don't work with C interface, I just use the tools under Linux and recently  have problem with the Mirax files generated by the new 3DHistech scanner. The new file format does contain some extra information that OpenSlide rejects or doesn't understand. I read in other mailing lists that people have successfully use 3DHistech converter (free download from 3DHistech site) to convert from MIRAX format to MIRAX format (same format conversion) and can successfully use the converted file with OpenSlide. The converter basically removes the extra overlapping information from the file, This is an extra step that needs to be done on the scanner PC. Is there possibility that OpenSlide library can be updated to avoid this conversion step, eg, skipping the overlapping information in the new Mirax format.

Regards,
Thomas

On 4/9/2020 11:21 PM, Terry Wilson wrote:
I am using the C interface to the windows version of OpenSlide.  I have successfully used it to for a number of different formats, however, the same code does not appear to be working for Mirax slides.  I am able to open the slide and see the various properties, however, anytime I try calling openslide_read_region, it always just fills in the buffer with 0's.  According to the documentation that is an indication of some error, however, calling openslide_get_error immediately after just returns a null error object.

I saw an older post from someone on this mailing list who seemed to have the exact same problem I am having with Mirax slides ... unfortunately they seemed to eventually get it working but had no idea what they changed to get it to work.  So I am looking for some suggestions on what to try.  I have tried retrieving all the data for the entire level 0 using a tile size of 512x512 and they all seem to come back with 0's.  I tried getting an entire higher level (level 6) with one call but same result.  I have also tried various different Mirax slides (including some of the demo ones from the OpenSlide site) The code is pretty simple:

// Try reading a single tile
// x, y values start at 0,0 and increase based on the tile size
int size = 512;

uint32_t *data = new uint32_t[size * size];

openslide_read_region(openslide, data, x, y, 0, size, size);
const char* err = openslide_get_error(openslide);
if (err != NULL)
{
    printf("error detected");
}

I used openslide_get_property_names / openslide_get_property_value to dump out all the various metadata values for the slides ... is there some value in this metadata that I need to be accounting for (I assumed that the OpenSlide library was already doing that)? Any suggestions on how to trouble shoot this problem would be greatly appreciated.

Regards,

Terry



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