Determining the actual scale factor ...

[Mitko Veta]

Image up-sampling increases the spatial resolution (although no new
information is added). Yes, my example was for the opposite situation.
I would expect that if you use low magnification and fine sampling, a
lot of the information in the image will be redundant (the image will
be blurry). Again, I might be wrong.

Mitko Veta



On Thu, Dec 2, 2010 at 3:39 PM, Hauke Heibel
<hauke.heibel at googlemail.com> wrote:
> On Thu, Dec 2, 2010 at 3:36 PM, Mitko Veta <mitko.veta at gmail.com> wrote:
>> As far as I can tell, the spatial sampling resolution is independent
>> of the magnification. For example, you can use a 40x lens, and for
>> some reason, decide to sample with a very coarse grid, which will
>> produce large pixel spacing. Or am I wrong?
>
> Sounds possible but how could you achieve a spatial resolution with a
> 20x objective which is normally only possible with a 40x objective?
> What you are describing is exactly the other direction, right?
>
> - Hauke
>