Reading file exported from Aperio ImageScope

Kent Johnson kent3737 at gmail.com
Tue Nov 6 10:22:42 EST 2012


Hi,

I'm trying to use OpenSlide (openslide-win32-20120908) to read a
partial-slide TIFF file exported from Aperio ImageScope. The file is
recognized as an Aperio file but the component images are not interpreted
correctly.

The first image is read by OpenSlide as the level 0 image. The second image
is a thumbnail. The third image is a label image and the fourth is a macro
image. OpenSlide ignores the second and third images and uses the macro
image as the thumbnail.

My expectation is that the second image would be used as the thumbnail and
the label and macro images would be included as associated images. Is this
something that should work correctly or am I stretching OpenSlide a bit too
much?

Below is the tiffinfo output for the image.

Thanks,
Kent

TIFF Directory at offset 0xc7563e
  Subfile Type: (0 = 0x0)
  Image Width: 3278 Image Length: 3026 Image Depth: 1
  Tile Width: 240 Tile Length: 240
  Bits/Sample: 8
  Compression Scheme: LZW
  Photometric Interpretation: RGB color
  Image Description: "Aperio Image Library v11.2.1 \r\n68340x43430
[49868,23390 3278x3026] (240x240) LZW;Aperio Image Library v8.0
.19\n69360x43530 [0,0 68340x43430] (240x240) JPEG/RGB Q=70|AppMag =
20|StripeWidth = 2034|ScanScope ID = PHUSCA-W20585|Filename =
13203|Title = none|Date = 01/03/07|Time = 14:09:11|User =
f92fa4e0-e189-4a07-bb9b-095c3f5bbde4|MPP = 0.4667|Left = 9.760571|Top =
22.175273|LineCameraSkew = 0.000000|LineAreaXOffset =
0.000000|LineAreaYOffset = 0.000000|DSR ID = 192.168.2.102|ImageID = 13203|O
riginalWidth = 69360|Originalheight = 43530|OriginalWidth =
68340|OriginalHeight = 43430"
  Samples/Pixel: 3
  Planar Configuration: single image plane
  Predictor: horizontal differencing 2 (0x2)
TIFF Directory at offset 0xd93922
  Subfile Type: (0 = 0x0)
  Image Width: 831 Image Length: 768 Image Depth: 1
  Bits/Sample: 8
  Compression Scheme: LZW
  Photometric Interpretation: RGB color
  Image Description: "Aperio Image Library v11.2.1 \n3278x3026 -> 831x768 -
;Aperio Image Library v8.0.19\n69360x43530 [0,0 68340x
43430] (240x240) JPEG/RGB Q=70|AppMag = 20|StripeWidth = 2034|ScanScope ID
= PHUSCA-W20585|Filename = 13203|Title = none|Date = 01
/03/07|Time = 14:09:11|User = f92fa4e0-e189-4a07-bb9b-095c3f5bbde4|MPP =
0.4667|Left = 9.760571|Top = 22.175273|LineCameraSkew = 0
.000000|LineAreaXOffset = 0.000000|LineAreaYOffset = 0.000000|DSR ID =
192.168.2.102|ImageID = 13203|OriginalWidth = 69360|Origina
lheight = 43530|OriginalWidth = 68340|OriginalHeight = 43430"
  Samples/Pixel: 3
  Rows/Strip: 3
  Planar Configuration: single image plane
  Predictor: horizontal differencing 2 (0x2)
TIFF Directory at offset 0xdb0ede
  Subfile Type: reduced-resolution image (1 = 0x1)
  Image Width: 356 Image Length: 320 Image Depth: 1
  Bits/Sample: 8
  Compression Scheme: LZW
  Photometric Interpretation: RGB color
  Image Description: "Aperio Image Library v11.2.1 \nlabel 356x320"
  Samples/Pixel: 3
  Rows/Strip: 7
  Planar Configuration: single image plane
  Predictor: horizontal differencing 2 (0x2)
TIFFReadDirectory: Warning, exported from ImageScope 13203.tif: unknown
field with tag 347 (0x15b) encountered.
TIFF Directory at offset 0xdbcd6c
  Subfile Type: reduced-resolution image (9 = 0x9)
  Image Width: 640 Image Length: 246 Image Depth: 1
  Bits/Sample: 8
  Compression Scheme: JPEG
  Photometric Interpretation: RGB color
  YCbCr Subsampling: 2, 2
  Image Description: "Aperio Image Library v11.2.1 \nmacro 640x246"
  Samples/Pixel: 3
  Rows/Strip: 16
  Planar Configuration: single image plane
  Tag 347: <unsupported data type in TIFFPrint>
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