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Greetings, list!<br>
<br>
This is Alexandre Khalrmov. I am going to develop support<br>
for ZVI file format.<br>
<br>
Please, give me links to sample files that I can download<br>
and play with. I'm currently looking for a file with a considerable<br>
width and height, to see how it is stored. My bet is that they<br>
are split into smaller rectangles, but I need to see which<br>
meta data is involved, for the images I have now are small <br>
enough to fit into one chunk. Also, I need small files with<br>
different pixel formats.<br>
<br>
Hopefully all those files are within 100-200 megabytes size, unless<br>
bigger size is really necessary to prove a concept.<br>
<br>
Please, also, specify the usage rights for each image. Most<br>
importantly: can this image be used as an example to put on<br>
OpenSlide website?<br>
<br>
I will be busy with other things till about Thursday, so, no rush.<br>
So far, I'm just collecting info. And even after that it won't be
too<br>
late.<br>
<br>
Basically, it's a OLE2 compound document storage format, same as<br>
the one used for Office 97 documents. The file I had a chance to
play<br>
with has several focus planes, (please correct my terminology here),<br>
and also each image has 3 channels: one for Hoechst 33342, one for<br>
Cy3 and one for FITC. Each channel is a separate greyscale image,<br>
however, inside Zeiss' ZEN software, those images can be viewed<br>
as blue, red and green channels respectively, or as a colored image<br>
combining those channels into one. My question is, do I need to<br>
be able to output those channels separately, or should I only care
about<br>
the resulting full-color image? Is there ever more than 3 stains
used?<br>
<br>
Also, I've noticed that this has been mentioned by my colleague <i>Agelos
Pappas:<br>
</i>for multiple focus planes images, I think it would be important<br>
to be able to choose the plane to display. I understand, that would
require<br>
change in the API, but this is an important feature! What's the use
of<br>
all this expensive high-resolution imagery if the object is out of
focus or<br>
totally invisible?<br>
<br>
Big thanks go to <i>Benjamin Gilbert</i> for maintaining such an
important project.<br>
I mean, aren't we all going to benefit from medical research, and
the situation<br>
with all these proprietary image formats for healthcare is just
something that<br>
I can't understand: this is just plain wrong.<br>
<br>
Best regards,<br>
<br>
-Alexandre Kharlamov<br>
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