Hi openslide folks, <br><br>I have a question for extracting Whole Slide Image by Using OpenslideAPI.<br><div><br></div><div>I called below function to extract image from whole slide image. (.svs)<br></div><div><br></div>
<div>openslide_read_region(osr, buf, startCoordinateX, endCoordinateY, 0, tile_w, tile_h);</div>
<div><br></div><div>For testing, </div><div><br></div><div>I execute command like as follows. </div><div><br></div><div>command: (region5 is the command that I modified ... it's exactly same as openslideTiler with different argument order. </div>
<div><br></div><div>Usage:</div><div>region inputFileName startCoordinateX startCoordinateY endCoordinateX endCoordinateY </div><div> </div><div>Actual command: </div><div>region5 TCGA-08-0352-01Z-00-DX2.svs 0 12425 500 12925</div>
<div><br></div><div>Input image : TCGA-08-0352-01Z-00-DX2.svs</div><div><br></div><div>As a result, </div><div>ppm file was generated (TCGA-08-0352-01Z-00-DX2.svs-0000000500-0000000500.ppm)</div><div><br></div><div>When I checked the starting coordinate (0, 12425) in the whole slide image by using aperio, </div>
<div>the coordinate seems different like (0, 13017) ...<br><br>I think that there is coordinate difference bug.<br><br>For example, based on theory, If i want to extract point (X, Y) from the whole slide image, <br>I need to extract the coordinate (X - (width/2), Y-(height/2)). <br>
<br>Starting point( 2000, 2000), extracting image width (500), extracting image height (500)<br><br>then, the starting point X, Y should be 2000 - (500 / 2) = 1750 <br><br>X, Y( 1750, 1750)<br><br>However, according to the testing, I need to set up extracting starting coordinate (X- (width/2)), Y- (height/2) - height). <br>
<br>I seem like I need to decrease coordinate height again. <br><br>X = 2000 - (500/2) = 1750<br>Y = 2000 - (500/2) - 500 = 1250<br><br>X, Y (1750, 1250)<br><br>Is this openslideAPI bug ? I am thinking that I can manually change the coordinate in the worst case.. <br>
<br>But, if somebody know the solution, it would be great help for me. <br></div><div><br></div><div>Thanks in advance. <br></div><div><br></div><div>Best,</div><div>Steve</div><br clear="all"><br>-- <br><div>***************************************************</div>
<div>Tae Wook (Steve) Oh</div>
<div><br></div>
<div>Intern for advanced biomedical database </div>
<div>Research & development</div>
<div>Center for Comprehensive Informatics</div>
<div>Emory University</div>
<div> </div>
<div>Email: <a href="mailto:TOH3@emory.edu" target="_blank">TOH3@emory.edu</a>, <a href="mailto:taewooko@gmail.com" target="_blank">taewooko@gmail.com</a></div>
<div>Web: <a href="http://student.gsu.edu/%7Etoh2" target="_blank">student.gsu.edu/~toh2</a><br>
<div>***************************************************</div></div><br>